![]() Try total protein normalization using stain-free technology instead of normalizing to a single housekeeping protein.If loading control expression varies with experimental conditions, try using another loading control Check that loading control expression is consistent across conditions using a secondary loading control.Loading control protein levels may vary between test and control conditions In addition to providing visual verification of transfer at every step, stain-free imaging enables total protein normalization in each lane, eliminating the need for housekeeping proteins as loading controls Visualize total protein on gels and blots using Bio-Rad’s Stain-Free Gels featuring our proprietary Stain-Free Technology.To verify protein transfer, stain the membrane with Ponceau S after blotting.To determine if there is residual, untransferred protein remaining on the gel, use a total protein stain on the gel after transfer.For example, Coomassie and colloidal gold are not compatible with downstream steps (see Bio-Rad Protein Stains and the Protein Stain Selection Guide) If planning to use the blot in downstream steps, make sure that your stain can be removed or is compatible with antibody detection. To determine whether the changes in loading control levels are due to differences in the amount of sample loaded, or if the differences are caused by variations in expression of the loading control proteins, use total protein stains (e.g., Ponceau S, Coomassie, colloidal gold, or SYPRO Ruby) to visualize proteins on gels and blots before and after transfer to determine relative protein loading. ![]() Check concentration of protein samples (e.g., using Bradford or Lowry protein assays).Initial sample quantitation (O.D., weight, cell count, etc.).Check that total protein levels are consistent:.Samples may have different amounts of total protein Variation observed among the loading controls in each lane ![]()
0 Comments
![]() #4: Bugatti Chironīugatti, never one to back down from a top-speed fight, built the Chiron as a successor to the already world-record-breaking Veyron Super Sport (below). As far as Hennessey goes, all eyes are now on the Venom F5 Revolution, which has the potential to shake things up on this list. Hennessey claims the Venom GT can reach a top speed of 278 mph if given a bit more room to do it. This beast can go from zero to 100 mph in 5.6 seconds (a world record) and hit 200 mph from a stop in only 14.51 seconds (also a world record). Sporting a twin-turbo 7.0-liter V8 producing a massive 1,244 horsepower, the Venom GT beat a world record set by the Koenigsegg Agera R as the fastest accelerating production car in the world when it did a run from zero to 186 mph in 13.63 seconds. This hypercar from Texas-based Hennessey is an impressive machine, to say the least. Koenigsegg said goodbye to the Agera in mid-2018 with two final examples named Thor and Väder. However, 11 lucky owners were able to (theoretically) hit the top speed of 277 due to checking the “1MW” special package when ordering theirs, which increases the ponies to 1,341. ![]() Powered by a 5.0-liter twin-turbo V8, the “normal” Agera RS produces 1,160 horsepower (on regular pump gasoline, mind you). There were only 25 examples of the Agera RS produced. It’s an Agera R at heart, with some of the advanced tech of the One:1 and some of the Agera S sprinkled in for good measure. The Koenigsegg Agera RS is a hybrid of sorts (no, not that kind of hybrid). The SSC Tuatara is powered by a twin-turbocharged 5.9-liter V8 producing 1,350 horsepower using 91 octane gas and 1,750 horses if you slip in some E85 juice. But that record wasn’t official, apparently, so they went back and did another run in January 2021, this time officially grabbing the record at 282.9 mph. On October 10th, 2020, the SSC Tuatara managed to claim the title of the world’s fastest production vehicle by clocking an average run of 316.11 mph (508.73 kph), also claiming the title for the first production car to break the 500 kph barrier. ![]() ![]() Created by American supercar maker SSC (formerly Shelby SuperCars, no relation to Caroll Shelby), the Tuatara not only beat the next fastest supercar on the list but blew it out of the water (or salt flats, as it were.) We’ve been hearing about SSC’s Tuatara since at least 2011. All the cars you would expect are here, ranked from fastest to slowest (though calling any of these slow is blasphemy.) And to clarify, yes, all of these cars are street-legal. Here is a list of the 10 fastest production cars in the world ranked by top speed. It takes lots of engineering genius to move so quickly down a strip of pavement without falling apart. Zero to 60 is probably the most realistic, everyday performance benchmark, but there’s no better ultimate ranking factor than top speed – the most no holds barred, balls-to-the-wall stat a supercar can have. ![]() ![]() ![]() At an array size of 10000 in_array() is a whole. Interestingly enough as the array sizes grow the in_array function becomes faster in average total runtime. At an array size of 1000 array_search takes on average 1.346 seconds in total time compared to the 1.41144 seconds for the in_array function. With smaller array sizes the php in_array function is just slightly worse in total runtime. Runtime Comparison Between In_Array() Function and Array_Search() Function Confusing these two functions can become time consuming if your array does not have keys and you are moving back and forth between functions. The key difference between the in_array() function and the array_search() function in PHP is the in_array() function just checks if the given value exists at all in the array and returns a boolean, whereas array_search() returns the key after searching the array. ![]() What's the difference Between PHP In_Array() Function and Array_Search() Function? It allows you to pass the same parameters to find the $needle in the $haystack as before. If you want to check the keys in an associative array the best option is actually to use the array_key_exists() function instead. PHP In_Array() Function To Find a Key In An Arrayīy default the in_array() function only checks if a value exists in an array compared to the elements of the array. ![]() Even when using array_map to handle case insensitivity the in_array() function searches $haystack for $needle using the same process as before. This function checks if a lowercase string value exists in an array by using an array_map when passing the $haystack in. Inputs: mixed $needle, array $haystack, bool $strict = false ![]() ![]() ![]() If you have cancelled the Billing Agreement and you would like to use PayPal again on Grailed, you must re-add PayPal as a payment method through the checkout page on your next Grailed purchase. ![]() You will no longer be able to use PayPal as a payment method on Grailed if you cancel the Billing Agreement through your PayPal Settings. Sadly, theres little you can do if you dont want a transaction going through PayPal. PayPal doesnt allow recurring payments unless you have credit cards or bank accounts linked to it, and they need to have enough funds as well. The Billing Agreement allows you to complete a purchase on Grailed with PayPal without having to log into your PayPal account for each purchase. If you already have an account, all you need to do is sign in. Once that is done, you can connect your new account when sending a new Binding Offer on Grailed. ![]() Please note that you will need to confirm the change via email, which will be sent to the email address associated with your Grailed account.įor buying, you can change your PayPal email address during the checkout process.įor Binding Offers, you can change your PayPal account by logging into PayPal and following their guide to cancel pre-approved payments. Please Note: A billing address must be input before a payment method can be added. For selling on Grailed, you can change your PayPal account by clicking here or navigating to your Settings page. Assign ad accounts to this payment method. Updated Fact checked We compare the following brands If you’re a regular online shopper, then chances are you’ve used PayPal. ![]() ![]() ![]() The Granular Permission System allows to manage Carl Bot commands in bulk and add global rules (rules that apply to all channels).Īnd most importantly, this smart system allows keeping all commands that look spammy in one designated channel. This feature is designed to give each command its own set of rules to follow. There’s also a self-destructing message whenever needed. You can block (blacklist and whitelist) them instantly and improve Discord security.Ĭarl bot comes in different modes, including reversed, unique, binding, verify, temporary and more, thus giving multiple roles with an individual reaction. The Reaction Roles includes 250+ roles, so you have many options to choose from.Īlso, it’s worth mentioning that this feature can help you deal with spammers. For instance, you can select the “salute” emoji to convert community members into moderators. You can choose an emoji that will work as a trigger for this role. ![]() Let’s say you want to reward the most dedicated members by allowing them to moderate chat messages. The roles can be assigned to members based on the emojis they are using. By using the Reaction Roles feature, you can set roles for community members and increase engagement. #1 Reaction RolesĪs a server owner, you can delegate a part of your responsibilities to other qualified members of your community. Here is a list of features that makes Carl Bot extremely useful for Discord server owners.
![]() ![]() The constant source of water ensures that wildlife viewing will be exceptional during all seasons. The second thing that makes the park so special is the 22 km of river running through the center. ![]() This of course equals exceptional viewing and photography opportunities. This means that we can be in very close proximity without disturbing them. Because there has been no hunting at the preserve for nearly 60 years and the only vehicles that traverse through the bush are a handful of Land Rovers (which do not interact with the animals), the animals at the preserve do not see the vehicles as anything outside of their natural habitat. ![]() We will drive off the road, through the river, under the trees and over the trees. Unlike the public national parks, we are not confined to staying on paved roads. With a maximum of 46 guests on the property at any given time, we pretty much have the place to ourselves. So what makes this safari so special? The preserve we visit is the largest and oldest "big five" game preserve in South Africa. What other people see during their entire safari, we often see in one day and from very close proximity. Needless to say, the wildlife viewing is unparalleled. We do not disclose the location because it is very exclusive and making reservations often takes us up to two years. Our destination is constantly counted as the world's top rated wildlife viewing destination by Conde Nast Traveler. This is the ideal time to visit because it is after winter and before the heavy rains of summer (remember, their seasons are opposite of the U.S.). Safaris take place once per year during mid-late November. The safari takes place in the north-eastern region of South Africa, in the province of Mpumalanga. It's little wonder that nearly half of our guests are repeat customers. The benefit of traveling with us is that we take the guess work out of where to go and we take care of all the little details to make your African adventure worry free. Africa is a large continent and the choices for a safari may seem overwhelming. For plenty of other great ideas to add to your itinerary, browse our Myrtle Beach vacation deals.We're happy you are considering traveling to Africa with the staff from the Myrtle Beach Safari. Broadway at the Beach is one major sight you need to see here.Check out our handy Myrtle Beach things to do page, too. As soon as you arrive, strike up a conversation with one or two of the 31,000 people who call this place home. Want to know what Myrtle Beach has to offer? You can't go past insider knowledge.With most things taken care of, our All-Inclusive hotels in Myrtle Beach are the perfect solution. Dread endlessly pulling your wallet out while on vacation? We know how you feel.Compare prices and amenities and take your pick! Condos, like Myrtle Beach 303A Villa Villa 6 and Myrtle Beach 301B Villa Villa 4, are also popular property types here.Browse our Myrtle Beach beach hotels and see what's available. Staying near the water means mesmerizing vistas and long walks along the beach at sunset.Things You Should Know About Myrtle Beach Many people also book Myrtle Beach vacation deals closer to Myrtle Beach Beaches, another excellent option. Litchfield Beach, located 19 miles (30 km) from the city center, is a great choice for those who are looking for convenience. Whatever you do, your trip to Myrtle Beach is sure to be a blast. Search around for colorful seashells or sit by the shore and listen to the soothing sound of the surf. Whether you're heading off alone or with the whole family, you're sure to find plenty of relaxation on your Myrtle Beach vacation. ![]() ![]() ![]() Therefore, “SEATTLE” or “seattle” can't be used in the password. For example, if the Windows Live ID secret question is Mother's birthplace, and “Seattle” is the answer, the password can't contain Seattle. Password and the User Password I am unable to link another email address. The password can't contain the same word that is the answer to the Windows Live ID secret question that helps you reset your password if you forget it. I have tried using both Microsoft Exchange and Microsoft Exchange Online and it. For example, if the email address is the password can't contain “tide” or "TIDE." Use business-class email through a rich and familiar Outlook experience you can. Outlook is the email and calendar product for Microsoft 365. Faculty, staff, and students have access to Office 365 as members of the. Office 2013 include applications such as Word, Excel. Enjoy a familiar user experience on iOS, Android, and Windows mobile devices. Stay more connected and productive with Outlook mobile apps. The maximum password length is 16 characters.Ĭannot contain any of the following items:Īccount name or part of your email address. Microsoft 365 Online lets you access apps like Outlook, OneDrive, SharePoint, Teams, Word. With 1 TB of OneDrive cloud storage, you can back up, share, and coauthor workbooks from any device. The minimum password length is 6 characters. Siga uno de estos procedimientos: Para activar el reenvío, seleccione Iniciar reenvío, introduzca la dirección de correo electrónico del reenvío y seleccione Guardar. Towards the end of last week, Microsoft announced that it will replace the Mail and Calendar apps in Windows with the new Outlook. En el menú Opciones, elija Correo > Cuentas > Reenvío. A Microsoft account does not need a Microsoft email The email address used to sign into your Microsoft account can be from, , Gmail, Yahoo, or other providers. Microsoft 365, Microsoft Outlook, Office 365. Outlook is the Microsoft 365 application utilized for mail and calendaring at the University of Nebraska System. The password can contain the following ASCII text characters: ` ~ ! # $ % ^ ![]() “After they receive the blue envelope they will have 45 days to respond, and that's when they really need to take action,” she said. “Until they are up for renewal, until they complete the redetermination process, they are going to be covered by MassHealth.”įor those who do not qualify for auto-renewal, Gonzalez said they should update their contact information through MassHealth and be on the lookout for blue envelopes arriving in the mail which contain instructions for redetermination. “The message really is that even though the health insurance landscape is extremely complicated to navigate, Massachusetts has a lot of programs and has a lot of opportunities for people to have access to health insurance,” Gonzalez said. Maria Gonzalez, the MassHealth Redetermination Campaign Director at Health Care For All, a nonprofit advocacy group focused on promoting health equity and fair access to medical coverage, said the goal is not to kick anyone off insurance. “There is no need to panic, there are windows built into the redetermination process so that people don't lose coverage immediately,” Cyr said. Julian Cyr, D-Truro, said about half of MassHealth members are expected to be automatically renewed. These calls will take 90 to 120 minutes, "but it's a start" at making the process more accessible, he said.īeginning in May, MassHealth officials will begin publishing a public dashboard that will show where the people who are losing MassHealth coverage live, their demographics, and the reasons behind lost coverage. The agency cut the long MassHealth application by about 75 percent for applicants over 65 years old, Levine said, and next month they will begin allowing seniors to do their applications over the phone. Levine said MassHealth is looking to make the application process easier and remain transparent throughout the redetermination process. The agency is ramping up its workforce across the board, Levine said, including doubling the amount of customer service representatives in call centers to 320, hiring an additional 100 workers to determine eligibility, 80 to 100 temporary employees to process applications, and using grants to scale up the certified application counselor workforce. MassHealth is boosting its workforce to help deal with redetermination of eligibility ![]() Maura Healey's fiscal 2024 budget proposal, Assistant Secretary for MassHealth Michael Levine said that the public insurance program is preparing for the onslaught of work checking the eligibility of those 2.3 million enrollees, and helping those who no longer qualify transition to other coverage.Īs the state struggles with a labor crunch, MassHealth hopes to double its call center workforce capacity in the next two months to deal with determining eligibility. At a Joint Ways and Means Committee hearing on health care investments in Gov. ![]() ![]() ![]() He developed the techniques for use of this device in newborn infants and has treated babies from all over the world. He recently evaluated a new artificial cornea device for implantation in cases of corneal blindness refractive to traditional keratoplasty and defined the indications for this keratoprosthesis in over 300 cases. Aquavella is an internationally recognized corneal surgeon who has been at the forefront of cornea research for decades. University of Rochester Medical Center Newsĭr. Links to ArticlesĪrtificial Cornea Offers Better Results for Infants, Some Blind Patients The team has performed the procedure on infants as young as 6 weeks old. Aquavella and the Eye Institute team provide hope to parents of children with blindness, including those with Peter's anomaly. The concept of utilizing an artificial cornea originates from the 18th century when it was first proposed to substitute a piece of glass to substitute for the scarred cornea. When a natural cornea transplant is not a good option more and more surgeons are recommending the keratoprosthesis to restore vision. Cornea transplants may fail due to rejection, and may yield poor results in certain cases. This method involves suturing natural cornea tissue from a donor eye to replace the scarred one or replacing a specific layer of the cornea using one of several sutureless procedures. Additionally, your age and the thickness of your cornea will also be taken into account when considering your candidacy for CXL.For many patients traditional cornea transplants work well in eliminating blindness due to cornea disease. Who is a candidate for corneal collagen crosslinking?Īn ideal candidate for CXL will have keratoconus progress for the past 12 -14 months. You may experience blurry vision and sensitivity to light at first, but your vision will return and possibly improve over time. Patients who may experience the most improvement in vision are usually older in their 50s or 60s and have keratoconus. Yes, corneal collagen crosslinking can improve your vision by improving the shape of the cornea. Does corneal collagen crosslinking improve your vision? If necessary, corneal collagen crosslinking can be repeated if the treatment is not effective. This also depends on how old the patient is when they undergo corneal collagen crosslinking. While we don’t know precisely how long corneal collagen crosslinking will last, the results may be permanent in some cases. The effects of your corneal crosslinking will last for many years. How long does corneal collagen crosslinking last? It is the only treatment available to halt progressive keratoconus from getting worse and can help to prevent a corneal transplant. CXL uses eyedrop medication and ultraviolet (UV) lights to strengthen the tissues in your cornea and stop them from bulging out. ![]() In most cases, complications of a cornea transplant can include an eye infection, a rise of pressure within the eyeball which can lead to glaucoma, or retinal problems like retinal detachment or swelling.Ĭorneal Collagen Crosslinking, or CXL, is a treatment method for keratoconus. This rejection will require immediate medical attention to either get another cornea transplant or to prescribe medication. There is a possibility for your body’s immune system to reject the donor’s cornea. ![]() How serious is a cornea transplant?Ī cornea transplant is a relatively safe procedure, however, like any surgical procedure, there is a risk of complications. You can also expect to use steroid or antibiotic eye drops daily for several months to a year. Following your procedure, it’s important not to rub your eyes, avoid intense exercise or heavy lifting, avoid manual labor work for 3-4 months and avoid contact sports and swimming until your doctor advises you to. Following your cornea transplant, you can expect to treat your eyes very carefully. The recovery process for a corneal transplant can last anywhere from a couple of weeks to a year. How long does it take to recover from a corneal transplant? ![]() ![]() Researchers have identified 1,159 manta rays at Tofo. This gives you a longer window to find a few. However, because the mantas in Tofo are cleaning shark wounds rather than parasites, a longer amount of time at the cleaning station is needed. ![]() To explain, manta rays normally spend about an hour at a cleaning station. In reality, these attacks are the reason Tofo is a reliable destination for manta ray diving. Approximately 75% of these mantas are female and many have suffered from shark attacks. This reliable food source attracts nearly 900 reef mantas and 100 oceanic mantas a year. Here’s a hot tip: consider a night dive in Palau, since the island’s manta population tends to be more active after sunset.īecause of unique current patterns off the coast of Mozambique, a 124-mile (200-kilometer) stretch of plankton-rich waters has been created. Divers generally report the best manta ray interactions during these months. Ready to pack your bags? Book your trip from October to May. ![]() Other famous dive sites are Stadium and the Dome. Sightings are almost guaranteed in the area at high tide because there are three different cleaning stations in the dive path. Devilfish City is a second, essential dive for anyone looking for mantas. The German Channel also happens to include a manta ray cleaning station. These are perhaps more visible due to the growing popularity of the dive site known as the German Channel, considered one of the top three dive sites in Palau. The manta population is estimated to include around 350-450 individuals. It’s also recently had 80% of its surrounding waters designated as a Marine Protected Area in January 2020. Previously overshadowed by Yap in terms of manta ray diving, Palau is now recognized as one of the best and most consistent areas for seeing manta rays. ![]() |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |